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The Walvis Ridge system consists of a series of seamounts, ridges, and plateaus formed during the opening of the southern Atlantic Ocean since ~135 Ma. International Ocean Discovery Program Expeditions (IODP) 391 and 397T drilled six sites along the length of the hotspot track to understand the magmatic processes associated with evolving plume-ridge systems. The oldest drilled segment of the ridge system – Frio Ridge – extends from the Etendeka flood basalts in Namibia westward into the Atlantic Ocean. Site U1575 is on the Frio Ridge and is the closest site to the African continent. The site drilled 118.9 m of igneous basement with 70.7 m (59.5%) of recovery. The recovered core consisted of alternating sequences of submarine pillow lavas and sheet flows, some of which were massive (up to 21 m thick). Preliminary major and trace element data demonstrate the basaltic lavas are fractionated (MgO = 4.8-6.4 wt. %) with modest TiO2 contents (1.5-2.7 wt. %). The upper 52 m of igneous section (214-267 mbsf) are geochemically consistent throughout the various eruptive styles. However, an abrupt compositional shift to lavas with lower incompatible element abundances (TiO2, Zr, Sr, Nb, La, etc.) from 274-311 mbsf demonstrates a clear shift in magmatic source contributions. Below this, the lavas return to compositions similar to the upper portion of the hole. Shipboard natural gamma radiation (NGR) and magnetic susceptibility (MS) measurements correlate with mineralogical and compositional changes. Specifically, decreases in NGR correlate well with decreases in K2O, Sr, Y, and Zr. MS is positively correlated with zones containing olivine. Trace element discrimination plots demonstrate a dual character: Ti-V relationships are strongly MORB-like while Th/Nb suggests the lavas have both MORB and plume characteristics, consistent with the formation of the Frio Ridge through plume-ridge interaction. Elevated Zr/Nb and Y/Nb values are also consistent with a hybrid source. The composition of this core contrasts sharply with cores recovered from the younger Guyot Province to the southwest. Sites U1578 and U1585 have episodes of higher TiO2 contents (>3.5 wt. %) with trace element signatures (e.g. low Zr/Nb & Y/Nb) indicative of a pronounced plume component, consistent with an intraplate setting for the formation of the Guyot Province. 

ABSTRACT Soil ecosystems harbor diverse microorganisms and yet remain only partially characterized as neither single-cell sequencing nor whole-community sequencing offers a complete picture of these complex communities. Thus, the genetic and metabolic potential of this “uncultivated majority” remains underexplored. To address these challenges, we applied a pooled-cell-sorting-based mini-metagenomics approach and compared the results to bulk metagenomics. Informatic binning of these data produced 200 mini-metagenome assembled genomes (sorted-MAGs) and 29 bulk metagenome assembled genomes (MAGs). The sorted and bulk MAGs increased the known phylogenetic diversity of soil taxa by 7.2% with respect to the Joint Genome Institute IMG/M database and showed clade-specific sequence recruitment patterns across diverse terrestrial soil metagenomes. Additionally, sorted-MAGs expanded the rare biosphere not captured through MAGs from bulk sequences, exemplified through phylogenetic and functional analyses of members of the phylum Bacteroidetes . Analysis of 67 Bacteroidetes sorted-MAGs showed conserved patterns of carbon metabolism across four clades. These results indicate that mini-metagenomics enables genome-resolved investigation of predicted metabolism and demonstrates the utility of combining metagenomics methods to tap into the diversity of heterogeneous microbial assemblages. IMPORTANCE Microbial ecologists have historically used cultivation-based approaches as well as amplicon sequencing and shotgun metagenomics to characterize microbial diversity in soil. However, challenges persist in the study of microbial diversity, including the recalcitrance of the majority of microorganisms to laboratory cultivation and limited sequence assembly from highly complex samples. The uncultivated majority thus remains a reservoir of untapped genetic diversity. To address some of the challenges associated with bulk metagenomics as well as low throughput of single-cell genomics, we applied flow cytometry-enabled mini-metagenomics to capture expanded microbial diversity from forest soil and compare it to soil bulk metagenomics. Our resulting data from this pooled-cell sorting approach combined with bulk metagenomics revealed increased phylogenetic diversity through novel soil taxa and rare biosphere members. In-depth analysis of genomes within the highly represented Bacteroidetes phylum provided insights into conserved and clade-specific patterns of carbon metabolism.